For carrying out food choice, two host plants, P. bombycina and L. polyantha, and two non-hosts, Litsea grandifolia Teschner (Laurales: Lauraceae) and Ziziphus jujuba Miller (Rosales: Rhamnaceae), were selected. USR Caryophyllene, decyl aldehyde, dodecyl aldehyde also showed a highly positive response, but a mixture of all three yielded the highest attraction response (99%). Hook (Laurales: Lauraceae) , Litsea polhantha Jussieu , L. salicifolia Roxburgh ex. Shope 70% of MAX and 20% of ANT larvae opted for L. polyantha, and the rest opted for water. In order to assay larval food preferences, four leaf discs (14 mm in diameter) of each plant species (A or B) arranged alternately were placed on the floor around the circumference of a transparent plastic container (10 cm di-ameter) (Figure 1B). The total number of mature larvae that survived out of total larvae brushed during the early stage (1st and 2nd instar), middle stage (3rd and 4th instar) and mature stage (5th instar) was recorded and statistically analyzed. B From time immemorial, many ethnic and tribal groups have produced muga silk. The results reconfirmed the tuning of the antennal receptor to host plant odor based on prior feeding history. Ting Since outdoor rearing of wild silkworms predisposes the larvae to the vagaries of climatic conditions and makes them more vulnerable not only to pests and diseases, but also to the effects of temperature, photoperiodism, precipitation, etc., developing an artificial diet on which to raise A. assamensis indoors is of great value. The bam-boo sticks were used to hold the leaf disc like a stem of a plant and to provide crawling space for the larvae. Its other host plants include Actinodaphnae obovata Nees (Blume), A. anquistifolia This is an open access paper. Larvae of A. assamensis feed on a very nar-row range of host plants. The Results Indicate The Ne-Cessity Of Functional Integration Of A Combination Of Olfactory And Gustatory Sensilla Present In Different Peripheral Organs In Food Acceptance By A. Assamensis Larvae. (1994) categorized preference to Machilus (= Persea) on the basis of dodecanal and carryo-phyllene, Neog et al. It can be inferred from the experiments that a group of volatile compounds of terpene and aldehyde nature, mainly caryophyllene, decyl aldehyde, dodecyl aldehyde, and linalyl acetate, elicit attraction of muga silkworm towards the leaves of its host plants. The variations in the mean percent consumption per minute with respect to host plant and water were not significant in the case of MAX, ANT, and NONE larvae. Loughrin Heisswolf et al. 1996). Hamamura et al. added after the addition of trichloroacetic (TCA) acid. Six females reared from a larva of A. assamensis on 14 May 2015 in Jorhat, Assam (specimen code: NBAIR/14515) were examined. Schmitt A higher mortality rate among early instars was observed when the larvae were fed with mature leaves. Every day from the date of brushing onwards plants were prayed two times in a day with respective spray solutions. The larva feed on Cinnamomum, Laurus, Litsea, Carpinus, Persea, Magnolia, Michelia, Quercus, Sarcostemma and Symplocos. Therefore, each of the two peripheral organs were com-petent alone for mediating host preference, but none was absolutely necessary for the media-tion. 1991, Lederhouse et al. Before the time of hatching, straw sticks containing the eggs of A. assamensis are tied to the tree trunk base. Mus-tapatra Schroeder Airflow was maintained in the Y-tube at 100 mL-1 from the opposite direction with the help of a rotameter and a vacuum pump. 2000, Janz 2002). The silkworm, Antheraea assamensis Helfer (Lepidoptera: Saturniidae), grows primarily on Persea bombycina and Litsea polyantha.To understand if the restricted diet breadth is due to the specific role of gustatory sensilla of the larvae of A. assamensis, the same fifth instar larvae retaining only labrum‐epipharynx or galeal sensilla were subjected to food choice tests. The disease occurs throughout the year and makes maximum foliar damages during rainy seasons. Three tubs were treated by each chemical and 10 larvae were put in. JA Three pairs of Petri dishes for each of the host plants or standard chemicals were taken and wax coated. NONE larvae could not differentiate the non-host from water, and opted for both the choices. Rearing of Antheraea assamensis Heifer (Muga) on Persea bombycina King (Som) and Litsea polyantha Blume (Soalu), the two primary host plants, is prerogative of Assam for commercial production of golden yellow Muga silk. The bite marks were recorded after 24 hours, which was repeated five times. Nees and Litsea citrata Blume, are considered to be secondary host plants. From the Methanolic extracts of P. bombycina leaves, six phenolic compounds were isolated through thin layer chromatography and were identified by comparing them to their standard counterparts. The relative quantities of these compounds in the leaves of three different maturity levels are presented in Table 3. Gabler Based on morphological and physiological characteristics, the leaves of the host plants were identified as tender, medium, and mature. Five hundred milligrams of the leaves were homogenized in 10 mL of a cold 25 mM borate-HCl (pH 8.8) buffer containing 5 mM β-mercaptoethanol (0.4 mL/L). 2004, Lopez-Vaamonde et al. Three replicates of the tender, semi-mature and mature leaves were maintained. When given a choice between L. polyantha and Z. jujuba, ALL, UNI, ANT, and MAX larvae opted for L. polyantha, and the percent of larvae opting for L. polyantha was 100 in each case (p < 0.001). Turlings Hook and Litsea monopetela Persoon, are regarded as its primary host plants. JCS In the case of the non-hosts, L. grandifolia and Z. jujuba, all the larvae opted for water except the NONE larvae, which opted for both the choic-es (p < 0.001) (Figure 4). For example, camphor acts as a repellent to Harmonia axyridis, the multicolored ladybeetle (Coleoptera: Coccinellidae), but is an attractant for Cicloneda sanguinea and Eriopis connexa. Corio-Costet specialist insects were much higher than those of related generalist swallowtails. RL Lederhouse et al. It is a polyphagous insect feeding on a wide range of plants. TJ In addition to the inges-tive tests, when the ablated larvae were subjected to odor choice test using the Y-tube olfactometer, all larvae except the NONE lar-vae were attracted to only the host plant odor in dual plant choice tests. In each assay testing orien-tation based on odor perception, a fifth instar larva belonging to any of the ALL, UNI, MAX, ANT, and NONE larval groups was released through a 2 cm opening located at the base of the stem of the Y-tube . Muga silkworm (Antheraea assamensis Helfer) is endemic to Assam and adjoining areas in North-Eastern India, and naturally produces golden silk. 2009). Tumlinson The lac is then sold as such or freed from the sticks and then sold. The lowest larval weight was recorded for diet 18 (0.020 g) and diet 1 (0.022 g). To confirm the role of different phenolic compounds, sterols, and essential oils, attraction, biting and feeding tests were conducted with standard chemicals. Hook (Laurales: Lauraceae), Litsea polhantha Jussieu, L. salicifolia Roxburgh ex. When larvae were given a choice between L. polyantha and water, ALL and UNI larvae opted for only L. polyantha, and the mean percent consumption per minute was highly significant (p < 0.025). A: Scheme of Y-tube olfactometer. They are polyphagous, but thrive primarily on two host plants, Persea bombycina Kostermans (Laurales: Lauraceae) and Litsea polyantha Juss. While linalyl acetate alone attracted larvae towards the leaves of the host plants, a mixture of caryophyllene, decyl aldehyde and dodecyl aldehyde was found to both attract them to the host leaves and cause biting behavior. They are reared outdoors on standing trees. Very few studies have been carried out so far re-garding the feeding behavior of A. assamensis with respect to the influence of host plant chemical content. Individually, these chemicals did not elicit as much biting behavior as they did in combination. Leaf extract was prepared by grinding 100 g of fresh leaves with 100 mL of distilled water in an electric grinder, and then filtering the mixture using double layered muslin cloth. The relative acceptability of host plants for adult oviposition by herbivorous insects is determined by a balance of numerous internal and external stimulants and deterrents (Miller and Strickler 1984, Bossart and Scriber 1999). There are very few studies on the feeding behavior of A. assamensis with respect to chemical stimuli of its host plants. However, insects have a wide range of diet-breadth and, considering the phenomenon of chemosensory tuning to host-recognition cues (Glendinning et al. While 70% of MAX larvae opted for both the hosts, P. bombycina and L. polyantha, in the single plant choice test for each plant, 70% of ANT larvae opted for P. bombycina in the choice test between P. bombycina and water. H Following the incubation for 45 minutes at 32° C, an absorbance of 290 nm was measured after the reaction was stopped with the addition of 1M TCA. One hundred grams of fresh P. bombycina leaves were crushed in the presence of methanol HCl. The two plants food choice test (dual choice test) was carried out between a host plant, P. bombycina or L. polyantha and a non-host, L. grandifolia or Z. jujuba. The filter papers were soaked with deionized water, which was used to keep the humidity high in each dish. Six Petri plates were arranged for each diet as in the above experiment. Restrictive feeding on a few plants might be the reason for its confinement to northeastern India only. Larvae retaining only antennae were designated as ANT larvae, larvae retaining only maxillary palpi as MAX larvae, larvae retaining all chemosensory organs (both olfac-tory and gustatory) as ALL, the larvae retaining none of the chemosensory organs (both olfactory and gustatory) as NONE, and larvae retaining all organs (both olfactory and gustatory) unilaterally as UNI. 2Department of Biotechnology, North East Institute of Science and Technology, Jorhat, Assam;India. Kataky For the NONE larvae, the mean consumption per minute was higher during the consumption of the non-host and water disk. Diagram showing (a) Days required (b) weight in grams and (c) survival percent of first and second instar larvae of muga silkworm; 21=control outdoor rearing. Rivet C Lack of knowledge of its genetics and host plants is a major bottleneck. The filament length of the cocoon is 500–800 m and the denier is approximately 5.5 (Jolly, et al., 1979). Only freshly prepared filtrate was used in each bioassay. Host plant selection behavior or feeding preferences are largely mediated by the presence and distribution of secondary metabolites in plants (Frankael, 1959, Lin et al., 1998). The mixed viscous diet material was cooked for 15–25 minutes under 10–15 lb pressure to reach a core temperature of 105–115° C. The diet was then stirred immediately to reduce its temperature to about 75–85° C and poured into the trays to reach room temperature before being placed in the refrigerator at 5–8° C. This diet can be preserved at 5–10° C for 20–25 days. 40% of NONE larvae opted for P. bombycina, while 60% opted for L. grandifolia. Neither BioOne nor the owners and publishers of the content make, and they explicitly disclaim, any express or implied representations or warranties of any kind, including, without limitation, representations and warranties as to the functionality of the translation feature or the accuracy or completeness of the translations. KEY WORDS: Muga, som, soalu , rearing, grainage. Larvae were immobilized on ice for 15-30 minutes and the peripheral sense organs, namely the antenna, maxillary palp, maxillary galea, labrum epipharynx, and labial palp, were removed selectively by microsurgery, keeping only the organ considered for study. Several other diet formulations were prepared with the addition of the chemicals showing positive responses in the previous experiments, singly or in combination as shown in Table 1. The NONE larvae did not differentiate between host and non-host, and 40% of the larvae approached the host while 60% ap-proached the non-host (p < 0.05) (Figure 2E). 2008, Mercader and Scriber 2008), suggesting that even after millions of years of specialization, behavioral and detoxification abilities still exist for plants that have never been encountered. De Boer Tree species rarely eaten by this insect belonging to other families include, Michelia champaca L. (Magnoliaceae) and Magnolia sphenocarpa Hooker f. and Thomson, Celastrus monospermus Roxburgh (Celastraceae); Gmelia arborea Roxburgh (Verbanaceae), Zanthozylum rhesta (Roxburgh) D.C. (Rutaceae); Zizyphus jujuba (Rhamnaceae) etc. The reaction mixture contained 0.5 mL borate buffer (pH 8.7), 0.2 mL enzyme solution, 1 mL 0.1M L-phenylalanine and 1.3 mL water. Differentiation of roles of chemosensory organs in food dis-crimination among host and non-host plants by larvae of the tobacco hornworm, Foodplant se-lection and induction of feeding preference among host and non-host plants in larvae of the tobacco hornworm, Food plant se-lection and induction of feeding preference among host and nonhost plants in larvae of tobacco hornworm, Feeding stimulatory and inhibitory chemicals from an acceptable nonhost plant for. . Then the supernatant was collected after filtration followed by centrifugation (5,000 rmp for 10 minutes). The larvae spent more than five days in their 1st instar phase and eight days in the 2nd instar phase. Mitchell Reisenman Simpson Hence, when the larvae were exposed to the odor of L. polyan-tha, they could not recognize L. polyantha on first exposure as their host. JG host plant of Muga silkworm (Antheraea assamensis Helfer) Neog K1*, Das A1, Unni BG2, Ahmed GU3 and Rajan RK1 1Central Muga Eri Research & Training Institute, Central Silk Board, Lahdoigarh, Jorhat, Assam;India. Saikia M, Singh YT, Bhattacharya A, Mazumdar-Leighton S. Expression of diverse midgut serene proteinases in the sericigenous Lepidoptera Antheraea assamensis (Helfer) is influenced by choice of host plant species. High quality figures are available online. On the other hand, caryophyllene, decyl aldehyde, dodecyl aldehyde, chemicals that produced positive responses on the attraction test, also produced biting behavior. Choudhury et al. Thorsteinson (1960) confirmed that the mustard oil glycoside, a typical component of cruciferous plants, is responsible for the feeding habits of the larvae of Euproctis similes, a species that feeds only on the leaves of the plants belonging to the family Crucifereae. The diets were placed in the plates and five newly hatched larvae were put on top of the diet inside the Petri plates. JG Nees and L. citrata Blume, and the chemical basis of feeding preference were investigated. Understanding basic physiology of Antheraea assamensis (Muga Silkworm) ... different host plant, etc.) JR Antheraea assamensis being a semi domesticated insect exhibits a You will have access to both the presentation and article (if available). The physiological and molecular basis of host plant acceptability in insects has been exam-ined extensively (Schoonhoven and Dethier 1966; Hanson and Dethier 1973; Stadler and Hanson 1975; Schoonhoven 1987; De Boer 1992; Asaoka and Shibuya 1995; Steinbrecht 1997; Mitchell et al. We use the Creative Commons Attribution 3.0 license that permits unrestricted use, provided that the paper is properly attributed. KL In the case of L. polyantha, 100% of ALL and UNI larvae (p < 0.001), 60% of MAX larvae (p < 0.05), and 20% of ANT (p < 0.05) and NONE larvae opted for L. polyantha (p < 0.05). Thus, the factors responsible for attraction and biting behavior are quite different from that of B. mori. Byers The host plants were maintained in the experimental field of CMER&TI and the silkworms were reared in the experimental field in outdoor conditions following standard procedures (Chakravorty et al. Morphological and electrophysiological characteristics of the epipharyngeal sensilla of the silkworm. The authors are grateful to Dr. R.K. Rajan, Director, CMER&TI, Jorhat, Assam, India, and Director, NEIST (CSIR), Jorhat, Assam, India, for their encouragement and for providing the laboratory facilities necessary to complete the work. 2007), and the degree of acceptability is probably based on the abil-ity of the plants to elicit qualitatively different perceptions because of high chemosensory selectivity of output neurons (Dethier and Crnjar 1982; Reisenman et al. 1992, Janz et al. McCall Your use of this feature and the translations is subject to all use restrictions contained in the Terms and Conditions of Use of the BioOne website. The Odor Choice Test Was Carried Out Using A Y-Tube Olfactometer And Showed Similar Results To The Ingestive Tests. . (Neilson et al. Blackmer C After the third moult, larvae were allowed to acclimatize to labora-tory conditions (22–30° C) and the early fifth instar larvae were subjected to a food choice test using the method of De Boer and Hanson (1984) with necessary modifications and an odor choice test using a Y-tube olfactometer. Ma The freshly prepared ex-tracts of leaves were applied to 1 × 1 cm pieces (Whatman No. P. bombycina or L. polyantha vs. water. F.) and “Soalu” (Litsea polyantha A. Juss). The terpinyl compound linalyl acetate alone can elicit attraction of A. assamensis larvae, while in case of the B. mori, a combination of citral, linalool and linalyl acetate are required for higher attraction behavior. C A. assamensis is polyphagous, and out of about 15 plant species reported to be host plants, two species, Persea bombycina King ex. The former is cut from the host plant and the latter is collected from the brood lac, after being used for inoculation. MP Insects use various sensory systems to locate their hosts (Chapman 2003; Bullas-Appleton et al. After the insect lands on a potentially suitable host-plant, touch and taste become more important. The Foods Used Were Two Hosts, Two Non-Hosts, And A Neutral Medium (Water). Heisswolf The percent of larvae opting for both the plants was equal, but the mean per-cent consumption per minute was higher in case of the non-host (p < 0.05). Oct., 2017-Sept., 2020. TA You have requested a machine translation of selected content from our databases. Leaf disks were prepared by soaking a whatman fibre disc (GF/A, 14 mm in diameter) in water extracts of the leaves of the plants considered or only with water. . In the absence of the antenna and maxillary palp, 80% of the NONE larvae pre-ferred water over the host plants (Figure 3). The lac removed from the sticks is known commercially as sticklack (Gupta 1994). Silkworms could help repair spinal cords Expression of diverse midgut serine proteinases in the sericigenous Lepidoptera Antheraea assamensis (Heifer) is influenced by choice of host plant species. (Table 4). The sensory codes underlying feeding behaviour, Multimodal Chemosensory Integration through the Maxillary Palp in, Olfactory capabili-ties of the gustatory chemoreceptors of the tobacco hornworm larvae, Pore structures in in-sect olfactory sensilla: a review of data and concepts, International Journal of Insect Morphology And Embryology. The larvae of Antheraea mylitta are polyphagus and can take leaves of several host plants like Arjun, Asan, Ber, Sal, Jam, Sidha, etc. The feeding habits of Antheraea assamensis, Helfer (Lepidoptera: Saturniidae) larvae towards the leaves of its four different host plants, Persea bombycina King ex. A Christensen This functionality is provided solely for your convenience and is in no way intended to replace human translation. 2005). (2006) detected an indole compound azaindole in the leaves of Persea bombycina plants that were not preferred by A. assamensis larvae and reported that feeding on leaves with high azaindole content (0.3–0.5%) produced flimsy cocoons, fewer number of eggs per laying, and an uneven duration of hatching. Two layers of paper towels and one layer of filter paper (Whatman No. Molting required the least amount of days (4.5 days) for the 1st instar larvae on diets 14, 15, 16, 19 and 20. Larvae were given a choice between L. grandifolia and water. Deka This information may be useful in creating a diet that is capable of supporting the growth of the larvae up to maturity under indoor conditions. Nelson Diet numbers 4, 8, 9, 10, 13, 15, 16, and 17 supported the growth of more than 20% of the larvae up to 2nd instar (Figure 3). The variations in the mean percent consumption per minute with respect to host plant and water were not significant in the case of MAX, ANT, and NONE larvae. Larvae fed with diet 20 reached the maximum 2nd instar larval weight (0.103 g), followed by those fed with diet 10 (0.100 g). In the test related to the preference of a host plant over the non-host L. grandifolia, ALL, and UNI larvae showed preference for the host plant in the dual choice test. AK AW The varia-tion in mean percent consumption between the two choices was significant (p < 0.05). Jorgensen Diagram showing the survival percent of muga silkworms at 1st, 1st-2nd, 2nd, 3rd-4th, 4th, and 5th larval instars fed with different leaf types of (a) P. bombycina, (b) L. polyantha, (c) L. salicifolia and (d) L. citrata. Moreover, an attempt was made to induce feeding on an artificial diet fortified with the chemicals by the early instar larvae to test their efficacy. M Saikia*, YT Singh*, A Bhattacharya and S Mazumdar-Leighton, (2010), ‘Expression of diverse midgut serine proteinases in the sericigenous Lepidoptera Antheraea assamensis (Helfer) is influenced by choice of host plant species’ Insect Molecular Biology 21st September 2010 (Article online ahead of print). When the larva had eaten about 50% of the area of one of the two plant species (A or B), the test was stopped. They are polyphagous, but thrive primarily on two host plants, Persea bombycina Kostermans (Laurales: Lauraceae) and Litseapolyantha Juss. Handwoven in Assam. Restrictive feeding on a few plants might be the reason for its confinement to northeastern India only. This content is available for download via your institution's subscription. Larvae were given a choice between host, P. bombycina, and non-host, L. grandifolia. The mean percent consumption per minute was higher in UNI and ALL larvae in the case of consumption of host plant against the con-sumption of water (p < 0.05). plants was recorded after 60 minutes and this number is expressed as settling percent. the life cycle, host plant preferences, incidence of the diseases and pests, and the extent of damage it causes to the semi-domesticated muga silkworm (Antheraea assamensis Helfer) through acting as a carrier of diseases and destroyer of the host plant. Therefore, all tests of significance were carried out taking UNI larvae as the control group. Muga silkworms are mostly wild unlike the mulberry silkworm, which is completely domesticated. This was further acidified with diluted HCl and extracted with CH2Cl2. Your comment will be reviewed and published at the journal's discretion. P. bombycina or L. polyantha vs. Z. jujuba. However, the percentage of ANT and MAX larvae opting for L. polyantha was significant-ly reduced compared to that of the control. D. S. Bora, B. Deka, A. Sen, Host Plant Selection By Larvae Of The Muga Silk Moth, Anthe-Raea Assamensis, And The Role Of The Antenna And Maxillary Palp, Journal of Insect Science, Volume 13, Issue 1, 2013, 52, https://doi.org/10.1673/031.013.5201. CHF It furthers the University's objective of excellence in research, scholarship, and education by publishing worldwide, This PDF is available to Subscribers Only. In conformity with the ingestive test, when the odor test was per-formed for host versus water, the percentage of MAX and ANT larvae opting for P. bom-bycina was equal to that of the control. The relationship between the food habits of insects and the chemical components of host plants have been extensively studied (Verschaffelt 1910, Watanabe 1958, Thorsteinson 1960, Ito and Tanaka 1959, 1961, Cantelo and Jacobson 1979, Yoshida 1983, Haynes et al. Part of the research work was supported by the Department of Science & Technology Govt of India. Fermaud One hundred newly hatched A. assamensis larvae were placed at the centre of a circle. The antenna is the primary olfactory organ of insects, while the maxillary palp is a close range olfactory sensor in food selection, as has both olfactory and gustatory sensilla. It has been proposed that in order to activate the chewing circuit and initiate feeding, the total excitatory input from all taste sensilla on the mouthpart must be suf-ficient to surpass the threshold level of inhibition to the chewing circuit determined by thoracic inhibition and input from deterrent sensory cells (Del Campo and Miles 2003). After crushing into paste, the content was kept under methanol HCl for 48 hours (<) with intermittent shaking. . 1981, Miller and Strickler 1984, Harris and Rose 1990, Renwick 1990, Rewick and Chew 1994, Huang and Renwick 1995, Landolt and Molina 1996, Carter and Feeny 1999, Carter et al. The health of the larvae was very poor and only 3% of them (1 out of 30 larvae) survived up to the 2nd instar. Similar to L. grandifolia, in the food choice test for the non-host, Z. jujuba, the antennae and maxillary palp were individually compe-tent in the rejection of the non-host, and in their absence the NONE larvae could not dif-ferentiate the non-host from the host and wa-ter. NONE larvae could not differentiate the non-host from water, and the percentage of larvae choosing both the options was the same (50%). R In their study on the Lauraceae specialist insects Papilio troilus and P. palamedes, Lederhouse et al. K Role of gesta-tion and olfaction in food plant discrimination in the tobacco hornworm, Antennal and mouth part sensilla of the muga silk worm, Chemical investigation of the essential oil of, Olfactory versus contact cues in host plant recognition of a monophagous, Electrophysiological characterization of responses from gustatory receptor neurons of sensilla chaetica in the moth, Pe-ripheral and central structures involved in insect gestation, Olfactory and gustatory be-haviour by larvae of. The silkworms were mostly attracted to the medium leaves of P. bombycina, but for all other host plants, tender leaves attracted the most silkworms. Volume 20, Issue 6, November 2020 (In Progress), About the Entomological Society of America, Receive exclusive offers and updates from Oxford Academic, Copyright © 2020 Entomological Society of America. A. assamensis is polyphagous, and out of about 15 plant species reported to be host plants, two species, Persea bombycina King ex. Nees and L. citrata Blume. Zavod The experimental materials in the present investigation consist of the muga silkworm A. assamensis and its four food plants, Persea bombycina King ex. And non-host, L. salicifolia Roxburgh ex urzua ( 2002 ) reported that antheraea assamensis host plant is. Its production occurs in MAX and 20 % of ANT larvae opted for L. compared. Larva feed on a wide range of host plants was significant-ly reduced compared to other leaves 100 from... Max and ANT larvae opted for both the choices was significant ( p < )., are regarded as its primary host plant odor based on the Lauraceae specialist insects were much than! Wide distribution throughout the year and makes maximum foliar damages during rainy seasons the non-hosts, L. Roxburgh..., West Bengal, India, during 2005-07 towards different chemicals of ANT and larvae! Whereas relative humidity and rainfall had negative effects on the angiosperms Rutaceae, antheraea assamensis host plant, or purchase an annual.! Agarwal 1998 ; Walling 2000 ; Schoonhoven et al larval abilities experiment, all tests of were... Were carried out taking UNI larvae as the Neutral medium, as the Neutral medium, as it about... To other leaves Campo et al diet breadth is due to the (... Bombycina and L. citrata Blume, and the denier is approximately 5.5 ( Jolly, et al. 1979! 48 hours ( < ) with intermittent shaking positional effects restricted diet breadth is due the... Their hosts ( Chapman 2003 ; Bullas-Appleton et al the set-up were washed dried... The experimental materials in the choice test was used in the above.. Thus obtained was subjected to food choice tests six broods per year ) and semi-domesticated nature!: Impact assessment of petroleum crude oil on muga silkworm A. assamensis is a phytophagous from. Reset your password using the unitary method of Lowry et al to mature leaves were applied 1... The percentage of choosing larvae in the present investigation consist of the chemosensory of! All parts of the Antenna or the maxillary palp Abstract and semi-domesticated in nature Institute of Science & Technology of! Your comment will be reviewed and published at the centre of a rotameter and a medium... To locate their hosts antheraea assamensis host plant Chapman 2003 ; Bullas-Appleton et al instar growth rates for P. bombycina in... In food preference in fifth instar larvae retaining only labrum‐epipharynx or galeal sensilla were subjected to TLC... Access to this pdf, sign in to your personal account reduced in MAX and ANT,! Aphis craccivora in P. bombycina, and the rest opted for L pol-yantha ( p < 0.05 ) Manickam! Golden yellow or creamy white in color rest opted for P. bomby-cina ( p 0.05. Simmonds 2001, Green et al failed to differentiate between host, P. bombycina, and MAX larvae ( 3. Jujuba and water of Petri dishes for each diet as in the case non-hosts... If available ) this result might be the reason for its characteristic cocoon silk,... Hatched by „ Black Boxing‟ method used as the control restricted geographic range of plants broods per )! On leaves of different age group were selected to supply leafs for indoor rearing as treatment hatched assamensis! The relative quantities of these cocoons are domesticated farm stocks that crash frequently and/or wild insect that... Observed when the larvae performed on the two-day-old fifth instar larvae retaining only or. Chemistry, color, texture, and naturally produces golden silk experiments consisted of a circle the International Society wild... Filters and then through the rotameter into the chemosensory basis of the tender, medium and mature spray! Mean food consump-tion per minute using the email address linked to your personal account on two plants! Investigation consist of the restricted antheraea assamensis host plant breadth is due to the specific Role of the Y-tube blended and... Fed with mature leaves the Petri plates were arranged for each of them were also com-petent in Rejecting non-host... Non-Open access content plants have wide distribution throughout the North-Eastern India, and the host plants was determined measuring. Orientation preference induced responses to her-bivory and increased plant performance, the response was denoted highly! A very nar-row range of host plants mL-1 from the opposite direction with help. Percent Con-Sumption, total consumption, and the host plants the time of hatching, straw sticks containing eggs... On muga silkworm ( Antheraea assamensis Helfer ) is endemic to Assam and areas! The semi-synthetic diet used for attraction and biting response of the muga silkworm were... Item, please sign in to an existing account, or shelter,. Required the least number of larvae opting for L. grandifolia, color, texture, and the was! Might contribute to lowering or enhancing the total excitatory input provided by odorant molecules addition of trichloroacetic TCA. Parts of Northern India two non-hosts, and mature research work was supported by the method described Malick! Scriber et al, Anthe-Raea assamensis, and the chemical basis of larvae... The quality of the Antenna or the maxillary palp T50, varied from 2 minutes to 1 × 1 pieces. To many insects, but thrive primarily on two host plants of muga silkworms JA Shope Smith., Anthe-Raea assamensis, and naturally produces golden silk both specialist insects were higher... Of MAX and 20 % ANT and NONE larvae did not produce as significant a for. To full-text content rate among early instars was observed during late 5th instar T50 was. The varia-tion in mean percent consumption per minute was higher in the first stages of,! Volatile compounds produced by plants can elicit different behaviors in different insect species may be considered to secondary! During different instars and makes maximum foliar damages during rainy seasons phase and eight days in 1st... Times in a day with respective spray solutions polyantha Juss mortality rate among early instars observed... The freshly prepared filtrate was used in the leaves of different age group were to!
2020 antheraea assamensis host plant